@article {1161902, title = {Systematic study of truffles in the genus Ruhlandiella , with the description of two new species from Patagonia}, journal = {Mycologia}, year = {2019}, abstract = {Ruhlandiella\ is a genus of exothecial, ectomycorrhizal fungi in the order Pezizales. Ascomata of exothecial fungi typically lack a peridium and are covered with a hymenial layer instead.\ Ruhlandiella\ species have nonoperculate asci and highly ornamented ascospores. The genus was first described by Hennings in 1903 to include the single species,\ R. berolinensis. Since then, mycologists have uncovered\ Ruhlandiella\ species in many locations around the globe, including Australia, Spain, Italy, and the USA. Currently, there are four recognized species:\ R. berolinensis, R. peregrina, R. reticulata, and\ R. truncata. All were found near\ Eucalyptus\ or\ Melaleuca\ trees of Australasian origin. Recently, we discovered two new species of\ Ruhlandiella\ in Nothofagaceae forests in South America. They regularly form mitotic spore mats directly on soil in the forests of Patagonia. Here, we formally describe these new species and construct the phylogeny of\ Ruhlandiella\ and related genera using a multilocus phylogenetic analysis. We also revise the taxonomy of\ Ruhlandiella\ and provide an identification key to accepted species of\ Ruhlandiella.}, author = {Nattapol Kraisitudomsook and Rosanne A. Healy and Mujic, Alija B. and Donald H. Pfister and Nouhra, Eduardo R. and Matthew E. Smith} }