First collection of the asexual state of Trichaleurina javanica from nature and the placement of Kumanasamuha

Abstract:

Ascomata of Trichaleurina javanica (Pezizomycetes) are encountered frequently in nature in tropical Asia. Its anamorphic state has been described previously as similar to Kumanasamuha. Our study describes the unusual anamorphic fungal specimen, MOZ170, collected from Gorongosa National Park, Mozambique. The fungal strain MOZ170 is identified using ribosomal DNA sequence data, its morphology is described, and morphological differences between the naturally growing anamorph and in vitro derived culture are compared. Phylogenetic placement of Kumanasamuha sundara was also determined using available data. The internal transcribed spacer (ITS) and partial large ribosomal subunit (LSU) were sequenced. Phylogenetic analyses of LSU supported MOZ170 as the anamorph of T. javanica, and revealed the proper placement of the type species of Kumanasamuha, i.e., K. sundara, within the Dothideomycetes. MOZ170 is characterized by its dark conidiophores growing in tufts, and conidia with curved, appressed crests and ridges. The comparison between naturally growing and in vitro grown cultures showed that the in vitro cultured anamorph had larger conidiogenous cells, larger conidia, and longer and more numerous lateral fertile branches compared to the fungus in nature. The present report represents the first anamorph collected from nature for this genus and one of the few natural collections of the anamorphic state within Chorioactidaceae with the exception of those of Desmazierella species.

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